Ces Urol 2015, 19(4):281-290 | DOI: 10.48095/cccu2015041

Establishment and characterization of multidrug resistant cell line model of urothelial carcinoma

Michaela Kripnerová¹, Pavel Dvořák¹, Martin Pešta¹, Jitka Kuncová², Tomáš Vlas³, Martin Leba⁴, Luboš Holubec⁵, Jiří Hatina¹

¹ Ústav biologie, Lékařská fakulta UK v Plzni, Plzeň

² Ústav fyziologie, Lékařská fakulta UK v Plzni, Plzeň

³ Ústav imunologie a alergologie, Fakultní nemocnice Plzeň, Plzeň

⁴ Katedra kybernetiky, Fakulta aplikovaných věd, Západočeská univerzita v Plzni, Plzeň

⁵ Onkologická a radioterapeutická klinika, Fakultní nemocnice Plzeň, Plzeň

Kripnerová M, Dvořák P, Pešta M, Kuncová J, Vlas T, Leba M, Holubec L, Hatina J. Establishment and characterization of multidrug resistant cell line model of urothelial carcinoma.

Major statement: New chemoresistant urothelial bladder cancer cell line as an experimental model for studying the mechanisms of multidrug resistance in advanced bladder cancer.

Aim: The aim of the study was to establish and characterize a new multidrug resistant urothelial cancer cell line.

Materials and methods: The parental cancer cell line BC44 was previously established from the large exophytic and better differentiated papillary part of a progressive tumor (pT4 G3) of a female patient. The daughter cell line BC44DoxoR has been derived by prolonged culture in increasing doxorubicin concentrations. Morphological response of parental and daughter cells after application of the individual drugs (doxorubicin, methotrexate, vinblastine, cisplatin, and gemcitabine) has been followed by phase-contrast microscopy. Cell viability has been determined using mitochondrial dehydrogenases assay and quantified by absorption spectrophotometry. Man-Whitney U Test (significance level α = 0.05) has been applied for statistical evaluation.

Results: The derivative cell line BC44DoxoR exhibited chemoresistance to all cytostatics tested. To the best of our knowledge, this is the first report of such a multidrug resistant urothelial carcinoma cell line.

Conclusion: The new multidrug resistant urothelial bladder cancer cell line could provide important new insights into understanding the therapeutic resistance of advanced bladder cancer.

Keywords: Chemotherapy, multidrug resistance, urothelial carcinoma.

Received: August 28, 2015; Accepted: November 4, 2015; Prepublished online: November 5, 2015; Published: December 21, 2015