78 / 100
324
Ces Urol 2016; 20(4): 317–325
ORIGINÁLNÍ PRÁCE
dardnímu vyšetření kvality spermatu. Je sice časově
náročnější než SCSA a zatímnemá ani mezinárodně
standardizovaný protokol, ani definovaný horní li‑
mit DFI, takže údaje získané na různých pracovištích
se často výrazně liší. Je to ale metoda dostatečně
citlivá a umožňuje analýzu i minimálního množství
buněk. V praxi by tak mohla významně přispět
k identifikaci příčin infertility i k posouzení vhod‑
nosti spermií pro techniky asistované reprodukce.
Poděkování
Podpořeno grantem IGA MZd ČR NT/13770
LITERATURA
1. Saleh RA, Agarwal A, Nelson DR, et al.
Increased sperm nuclear DNA damage in normozoospermic
infertile men: a prospective study. Fertil Steril 2002; 78: 313–318.
2. Nallella KP, Sharma RK, Aziz N, et al.
Significance of sperm characteristics in the evaluation of male
infertility. Fertil Steril 2006; 85: 629–634.
3. Agarwal A, Allamaneni SS.
Sperm DNA damage assessment: a test whose time has come. Fertil Steril
2005; 84: 850–853.
4. Lewis SE, Agbaje I, Alvarez J.
Sperm DNA tests as useful adjuncts to semen analysis. Syst Biol Reprod
Med 2008; 54: 111–125.
5. Agarwal A, Said M.
Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum
Reprod Update 2003; 9: 331–345.
6. Evgeni E, Charalabopoulos K, Asimakopoulos B.
Human spermDNA fragmentation and its correlation
with conventional semen parameters. J Reprod Infertil 2014; 15: 2–14.
7. Lin MH, Kuo-Kuang Lee R, Li SH, et al.
Sperm chromatin structure assay parameters are not related to
fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm
injection, but might be related to spontaneous abortion rates. Fertil Steril 2008; 90: 352–359.
8. Muriel L, Garrido N, Fernández JL, et al.
Value of the sperm deoxyribonucleic acid fragmentation level,
as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracy‑
toplasmic sperm injection. Fertil Steril 2006; 85: 371–383.
9. Singh A, Agarwal A.
The role of sperm chromatin integrity and DNA damage on male infertility. Open
Reprod Sci J 2011; 3: 65–71.
10. Coccuza M, Sikka SC, Athayade KS, et al.
Clinical relevance of oxidative stress and sperm chromatin
damage in male infertility: An evidence based analysis. Int Braz J Urol 2007; 33: 603–621.
11. Dvořák M, Matejovičová M.
Principy a využití kometové analýzy při detekci poškození DNA. Chem
Listy 2008; 102: 977–983.
12. Schmid TE, Eskenazi B, Baumgartner A, et al.
The effects of male age on sperm DNA damage in
healthy nonsmokers. Hum Reprod 2007: 22: 180–187.
13. Hughes CM, Lewis SEM, McKelvey-Martin V, et al.
A comparison of baseline and induced DNA
damage in human spermatozoa from fertile and infertile men, using modified comet assay. Mol Hum
Reprod 1996; 2: 613–619.
14. Evenson DP, Larson KL, Jost LK.
Sperm chromatin Structure assay: Its clinical use for detecting sperm
DNA fragmentation in male infertility and comparisons with other techniques. J Androl 2002; 23: 25–43.
15. Fernández JL, Muriel L, Rivero MT, et al.
The sperm chromatin dispersion test: a simple method for
the determination of sperm DNA fragmentation. J Androl 2003; 24: 59–66.
16. Peluso G, Palmieri A, Cozza PP, et al.
The study of spermatic DNA fragmentation and sperm motility
in infertile subjects. Arch Ital Urol Androl 2013; 85: 1–13.
17. World Health Organization (1999).
WHO laboratory manual for the examination of human semen and
semen-cervical mucus interaction, 4
th
edn. Cambridge University Press, Cambridge, UK, ISBN 9780521645997.